10/13/2023 0 Comments Slicer dicer demo![]() Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs. Two-metal active site binding of a Tn5 transposase synaptic complex. Lovell, S., Goryshin, I.Y., Reznikoff, W.R. Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis. Nowotny, M., Gaidamakov, S.A., Crouch, R.J. ![]() A general two-metal-ion mechanism for catalytic RNA. Stereochemical course of Escherichia coli RNase H. Krakowiak, A., Owczarek, A., Koziolkiewicz, M. Single active site catalysis of the successive phosphoryl transfer steps by DNA transposases: insights from phosphorothioate stereoselectivity. Integrating DNA: transposases and retroviral integrases. Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain. Nucleic acid 3′-end recognition by the Argonaute2 PAZ domain. Structure and conserved RNA binding of the PAZ domain. Structure and nucleic-acid binding of the Drosophila Argonaute 2 PAZ domain. The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes. Argonaute and RNA–getting into the groove. Biochemical identification of Argonaute 2 as the sole protein required for RNA-induced silencing complex activity. Purified Argonaute2 and an siRNA form recombinant human RISC. ATP requirements and small interfering RNA structure in the RNA interference pathway. Single-stranded antisense siRNAs guide target RNA cleavage in RNAi. Martinez, J., Patkaniowska, A., Urlaub, H., Luhrmann, R. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. ![]() The RNA-induced silencing complex is a Mg2+-dependent endonuclease. RISC is a 5′ phosphomonoester-producing RNA endonuclease. Ribonucleases H of retroviral and cellular origin. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage. Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity. Argonaute2 is the catalytic engine of mammalian RNAi. ![]() Crystal structure of Argonaute and its implications for RISC slicer activity. A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins. Fragile X-related protein and VIG associate with the RNA interference machinery. A micrococcal nuclease homologue in RNAi effector complexes. Argonaute2, a link between genetic and biochemical analyses of RNAi. Hammond, S.M., Boettcher, S., Caudy, A.A., Kobayashi, R. RNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila. RNAi-mediated targeting of heterochromatin by the RITS complex. RNA-directed de novo methylation of genomic sequences in plants. Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies. Inhibition of translational initiation by Let-7 microRNA in human cells. A role for the P-body component GW182 in microRNA function. MicroRNA-directed cleavage of HOXB8 mRNA. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells.
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